Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00066470" target="_blank" >RIV/00216224:14740/13:00066470 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Complementary matrix-assisted laser desorption/ionization and inductively coupled plasma mass spectrometry detection of single separation record
Original language description
The analyses of native metal-protein and metal-metabolite complexes by hyphenated techniques that combine separation with a specific detection is of growing importance. Using a liquid junction, capillary electrophoresis (CE) was coupled in the off-line mode to both matrix-assisted laser desorption/ionization mass spectrometry (MALDI) and substrate-assisted laser desorption inductively-coupled plasma (SALD ICP) mass spectrometry (MS). Here we present a new approach for an off-line multidetection platformfor metalloprotein/metallopeptide analysis of fractions collected from a single CE run. CE fractions are collected on a special target and analyzed consecutively with MALDI MS and SALD ICP MS. The concept is demonstrated on the CE-MALDI/SALD ICP MS analysis of rabbit liver metallothionein (MT) isoforms: The mixture of MT isoforms was separated in uncoated fused silica capillary (70 cm, 75um) using 20 mM ammonium bicarbonate buffer, pH 7.4 as background electrolyte.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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