Complementary MALDI MS and SALD ICP MS detection of a single separation record for metalloprotein analaysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00066467" target="_blank" >RIV/00216224:14740/13:00066467 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Complementary MALDI MS and SALD ICP MS detection of a single separation record for metalloprotein analaysis

Original language description

Metals play a crucial role in physiology and pathology of biological systems. It has been estimated that the metalloproteins encompass about one third of all proteins. A novel method for comprehensive multidimensional analysis of metalloproteins is presented here. This approach is based on an off-line coupling of a single micro-column separation run to both substrate-assisted laser desorption (SALD) inductively coupled plasma (ICP) mass spectrometry (MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI) MS. The effluent fractions are collected on a custom-designed Au-coated polyethylene terephthalate glycol (PETG) sample target that is compatible to both MS methods. The whole concept is demonstrated on analysis of rabbit-liver metallothionein (MT) isoform mixture. The MTs are separated by capillary electrophoresis (CE) coupled to MALDI MS/SALD ICP MS via a liquid junction interface and a sub-atmospheric deposition chamber.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů