MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073485" target="_blank" >RIV/00216224:14740/14:00073485 - isvavai.cz</a>
Result on the web
<a href="http://pubs.acs.org/doi/abs/10.1021/ac402941e" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/ac402941e</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ac402941e" target="_blank" >10.1021/ac402941e</a>
Alternative languages
Result language
angličtina
Original language name
MALDI MS and ICP MS Detection of a Single CE Separation Record: A Tool for Metalloproteomics
Original language description
In this work, a novel approach based on off-line coupling of a single run of capillary electrophoresis (CE) separation to both matrix-assisted laser desorption/ionization (MALDI) and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) mass spectrometry (MS) is presented. Using a liquid junction and subatmospheric deposition chamber, CE fractions were extracted from a separation capillary and collected as 20-nL droplets on a custom-built polyethylene terephthalate glycol (PETG) target plate coated with a 10-nm gold layer which guaranteed compatibility with both MALDI and SALD ICP techniques. The MALDI matrix solution was then added to the produced spots. After it was dried, the separation record was consecutively analyzed in MALDIMS and ICP MS instruments. Thus, both proteomic and metallomic information was obtained off-line from a single CE run. The concept was demonstrated by the analysis of a mixture of rabbit liver metallothionein isoforms.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Anal. Chem.
ISSN
0003-2700
e-ISSN
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Volume of the periodical
86
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
647-654
UT code for WoS article
000329548700068
EID of the result in the Scopus database
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