Multienzyme electrochemical array sensor for determination of phenols and pesticides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00012356" target="_blank" >RIV/00216224:14310/05:00012356 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Multienzyme electrochemical array sensor for determination of phenols and pesticides

Original language description

The screen-printed four-electrode system was used as the amperometric transducer for determination of phenols and pesticides using immobilised tyrosinase, peroxidase, acetylcholinesterase and butyrylcholinesterase. Acetylthiocholine chloride was chosen as substrate for cholinesterases to measure inhibition by pesticides, hydrogen peroxide served as co-substrate for peroxidase to measure phenols. The compatibility of hydrolases and oxidoreductases working in the same array was studied. The detection of p-cresol, catechol and phenol as well as of pesticides including carbaryl, heptenophos and fenitrothion was carried out in flow-through and steady state arrangements. In addition, the effects of heavy metals (Cu2+, Cd2+, Fe3+), fluoride (NaF), benzene anddimethylsulphoxide on cholinesterase activities were evaluated. It was demonstrated that electrodes modified with hydrolases and oxidoreductases can function in the same array. The achieved R.S.D. values obtained for the flow system were

Czech name

Multienzymové elektrochemické sensory pro stanovení fenolů a pesticidů

Czech description

Stotiskový čtyřkanálový sensor sloužil jako amperometrický převodník pro stanovení fenolů a pesticidů použitím tyrosinasy, peroxidasy, cholinesteras AChE a BChE. Byla studována kompatibilita těchto enzymů při imobilisaci na stejném sensoru. Detekované látky zahrnovaly kresol, katechol, fenol a pesticidy jako karbaryl, heptenofos a fenitrothion. Měření byla prováděna v průtočném i míchaném uspořádání. Doba stanovení byla 15 min a limity detekce v oblasti mikromolárních (fenoly) a nanomolárních (pesticidy) koncentrací.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F97%2F0924" target="_blank" >GA525/97/0924: Trace analysis of toxic PCB and pesticides using detection and separation methods based on antibody specifity</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

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Volume of the periodical

65

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

349-357

UT code for WoS article

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EID of the result in the Scopus database

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