Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00013673" target="_blank" >RIV/00216224:14310/05:00013673 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Amperometric screen-printed biosensor arrays with co-immobilised oxidoreductases and cholinesterases

Original language description

Amperometric screen-printed biosensor arrays for detection of pesticides (organophosphates and carbamates) and phenols have been developed. Cholinesterases (AChE and BChE), tyrosinase (TYR), peroxidases (SBP. soybean and HRP. horseradish) and cellobiosedehydrogenase (CDH) were combined on the same array consisting of one Ag/AgCl reference electrode surrounded by eight radially distributed working electrodes of either carbon or platinum. Mainly cross-linking with glutaraldehyde was employed for enzyme immobilisation. The substrates for the enzymes were acetylthiocholine for cholinesterases (ChEs), cellobiose for CDH and hydrogen peroxide for peroxidases. Hydrogen peroxide was generated in the presence of glucose by co-immobilised glucose oxidase (GOx).All measurements were performed in an electrochemical steady state system specially constructed for eight channel screen-printed electrode arrays. The achieved relative standard deviation values calculated for different enzyme substrates

Czech name

Amperometrické sítotisková biosensorová pole s koimobilisovanými oxidoreduktasami a cholinesterasami

Czech description

Amperometrické sítotiskové multielektrodové biosensory byly připraveny použitím cholinesteras (AChE a BChE), tyrosinasy, celobiosa dehydrogenasy a peroxidas (křenová a sojová) na jediném sensoru. Byly optimalisovány podmínky měření. Testované vzorky odpadních vod pak byly klasifikovány pomocí chemometrické multivariační analysy.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

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Volume of the periodical

528

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

9-19

UT code for WoS article

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EID of the result in the Scopus database

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