Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00012561" target="_blank" >RIV/00216224:14310/05:00012561 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/05:00021364
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods
Original language description
A new method has been developed for the monitoring of glutathione S-tranferase (GST) detoxification activity toward styrene oxide (SO). The enzymatic reaction was carried out directly in thermostatted autosampler vial and the formation of conjugates between glutathione (GSH) and SO was monitored by sequential MEKC runs. The determinations were performed in a 50 um fused silica capillary using 50 mM SDS in 20 mM phosphate 20 mM tetraborate buffer (pH 8.3) as a background electrolyte; separation voltage 28 kV (positive polarity), temperature of capillary 25 oC and detection at 200 nm. The method is rapid, able to be automated, and requires only small amount of samples, which is especially important in the case of GST isoenzyme analyses.
Czech name
Sledování aktivity glutathion-S-trensferasy vůči styrenoxidu pomocí elektromigračních metod
Czech description
Byla vypracována metoda pro sledování aktivity glutathion-S-trensferasy vůči styrenoxidu pomocí elektromigračních metod
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
12
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
1357-1362
UT code for WoS article
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EID of the result in the Scopus database
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