Proteomic analysis of iron and sulfur oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00012638" target="_blank" >RIV/00216224:14310/05:00012638 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Proteomic analysis of iron and sulfur oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry

Original language description

Acidithiobacillus ferrooxidans is a chemolithotrophic microorganism oxidizing ferrous iron and inorganic reduced sulfur substances. These bacteria are involved in formation of acid mine drainage and in biohydrometallurgic technologies. Both iron- and sulfur-oxidizing cells play a different role in oxidation of metal sulfides. Specific proteins can be expected for each type of iron or sulfur metabolism. For the above reasons, comparative analysis of protein composition of Acidithiobacillus ferrooxidans cells grown on elemental sulfur and ferrous iron was performed. Newly developed protocol involving two-dimensional gel electrophoresis with immobilized pH gradients, improved protein reduction, mass spectrometry protein identification and full genome information was applied. It resulted in more than 1300 protein spots displayed in broad and basic pH ranges, the best A. ferrooxidans proteome resolution to date. A comparative image analysis revealed that the proteome was significantly influ

Czech name

Proteomová analýza bakterie Acidithiobacillus ferrooxidans rostlé na síře a na železe pomocí imobilizovaných pH gradientů a hmotnostní spektrometrie

Czech description

Pro proteomovou analýzu bakterie Acidithiobacillus ferrooxidans byla optimalizována nová metoda za použití 2-D elektroforézy s imobilizovanými pH gradienty, hmotnostní spektrometrie a kompletní genomové informace. Byla detegována řada fyziologicky významných proteinů zapojených do transportu a metabolismu síry a železa.

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F04%2F1309" target="_blank" >GA525/04/1309: Bacterial oxidation of inorganic sulfur substances, environmental aspects</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Molecular & Cellular Proteomics 4, 8 (Suppl. 1)

ISBN

1535-9476

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

"S234"-"S234"

Publisher name

American Society for Biochemistry and Molecular Biology

Place of publication

Birmingham, USA

Event location

Mnichov, Německo

Event date

Aug 28, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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