Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00013899" target="_blank" >RIV/00216224:14310/05:00013899 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Evaluation of (GTG)5-PCR fingerprinting for characterization of enterococci isolated from bryndza cheese
Original language description
A group of previously well-identified enterococci isolated from a typical Slovakian sheep-milk product bryndza were characterized by the (GTG)5-PCR fingerprinting in order to evaluate discriminatory power of this method for identification and strain-typing. Analysed group consisted of E. faecium (n = 76), E. faecalis (n = 25) and E. durans (n = 10) strains. They were sampled from two bryndza-processing manufactories during three monthly samplings performed successively (April, May and June) in 2000 and2001. Isolated strains were characterized and identified by biotyping, ITS-PCR, ddlE.faecalis and ddlE.faecium PCRs. Species identity of representative strains selected from each (GTG)5-PCR fingerprint subcluster was confirmed by SDS-PAGE of whole-cell proteins. The (GTG)5-PCR fingerprint profiles obtained from E. faecalis strains were visually very close. Similarly, E. durans strains revealed nearly identical fingerprints. E.
Czech name
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Czech description
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Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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