Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014127" target="_blank" >RIV/00216224:14310/05:00014127 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Sensitive detection of organophosphates in river water by means of a piezoelectric biosensor

Original language description

A highly sensitive piezoelectric biosensor has been developed for detection of cholinesterase inhibitors.The inhibitor benzoylecgonine-1,8-diamino-3,4-dioxaoctane(BZE-DADOO) was immobilized on a monolayer of 11-mercaptomonoundecanoic acid (MUA) selfassembled on the gold surface of the sensor. The binding of high-molecular-weight cholinesterase to the immobilized cocaine derivative was monitored with a mass sensitive piezoelectric quartz crystal (quartz crystal nanobalance; QCN). In the presence of an inhibiting substance in the sample, the binding of cholinesterase to the immobilized inhibitor was reduced. The decrease of the rate of mass change was proportional to the concentration of free inhibitor in the sample. This way the affinity sensor followedanti-cholinesterase toxicity and the enzyme activity of ChE was not addressed. An assay for detection of organophosphates (OP) was optimized. Regeneration of the sensor surface was achieved with 1 mol/L formic acid, which enabled 40 meas

Czech name

Citlivé stanovení organofosfátů ve vodách prostřednictvím piezoelektrického biosensoru

Czech description

Byl vyvinut piezoelektrický biosensor pro detekci organofosfátů pomocí monovrstvy MUA-BZE-DADOO vážící cholinesterasu. Ke snížení vazby dojde v přítomnosti inhibitorů. Stanovení bylo testováno pro paraoxon, chlorpyrifos, chlorfevinfos a DFP.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

382

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

1904-1911

UT code for WoS article

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EID of the result in the Scopus database

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