Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00013580" target="_blank" >RIV/00216224:14310/05:00013580 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Piezoelectric affinity sensors for cocaine and cholinesterase inhibitors

Original language description

We report here the development of piezoelectric affinity sensors for cocaine and cholinesterase inhibitors based on the formation of affinity complexes between an immobilized cocaine derivative and an anti-cocaine antibody or cholinesterase. For both binding reactions benzoylecgonine-1,8-diamino-3,4-dioxaoctane (BZE-DADOO) was immobilized on the surface of the sensor. For immobilization, pre-conjugated BZE-DADOO with 11-mercaptomonoundecanoic acid (MUA) via 2-(5-norbornen-2,3-dicarboximide)-1,1,3,3-tetramethyluronium-tetrafluoroborate (TNTU) allowed the formation of a chemisorbed monolayer on the piezosensor surface. The detection of cocaine was based on a competitive assay. The change of frequency measured after 300 s of the binding reaction was usedas the signal. The maximum binding of the antibody resulted in a frequency decrease of 35 Hz (with an imprecision 3%, n = 3) while the presence of 100 pmol l1 cocaine decreased the binding by 11%. The limit of detection was consequently b

Czech name

Piezoelektrické afinitní sensory pro kokain a cholinesterasové inhibitory

Czech description

Byl vyvinut piezoelektrický afinitní biosensor pro stanovení kokainu a cholinesterasových inhibitor založený na vzniku komplexu mezi imobilisovaným derivátem kokainu benzoylecgonin-1,8-diamino-3,4-dioxaoktanem (BZE-DADOO) a acetylcholinesterasou. Imobilisace byla provedena pomocí monovrstvy merkaptoundekanové kyseliny. Detekce byla provádna kompetitivním zpsobem, limit detekce pro kokain byl 100 pmol/l, stanovení trvalo 15 min.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

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Volume of the periodical

65

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

337-342

UT code for WoS article

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EID of the result in the Scopus database

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