Electrochemical immunosensor for detection of Francisella tularensis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00015144" target="_blank" >RIV/00216224:14310/05:00015144 - isvavai.cz</a>
Alternative codes found
RIV/00000493:G41__/05:#0000032
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical immunosensor for detection of Francisella tularensis
Original language description
The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attachedthrough the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.
Czech name
Elektrochemický imunosensor pro detekci Francisella tularensis
Czech description
Byl vyvinut elektrochemický imunosensor pro rychlou detekci bakterie Francisella tularensis, patřící mezi biologické bojové prostředky.

Project
<a href="/en/project/OBVTUO2003001" target="_blank" >OBVTUO2003001: Imunosensors of CWA and BWA</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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