Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00013671" target="_blank" >RIV/00216224:14310/05:00013671 - isvavai.cz</a>

Alternative codes found

RIV/00000493:G41__/05:25152

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution

Original language description

Francisella tularensis is a pathogenic bacterium causing the serious disease tularemia and is potentially considered as a bioweapon agent. The piezoelectric immunosensor for F. tularensis (Ft) assay was developed using the quartz crystal microbalance (QCM) with covalently immobilized Ft antigen. Samples containing the bacterium were preincubated with a highly diluted anti-Ft IgM, and the mixture was injected to the flow cell containing the immunosensor. IgM alone as well as Ft cells alone were not giving any response, and therefore, it is assumed that the sensor responded to the IgM(x)-Ft(y) complex formed during the preincubation step. The effects of pH, time of preincubation, and IgM concentration were tested. This rapid method allowed us to detect 5(.) 10(6) Ft cells/mL. RSD of measurements was near 1%. The total time of one analysis was 35 min, and one sensor was used for more than 30 times without losing sensitivity.

Czech name

Piezoelektrický imunosensor pro detekci Franciselly tularensis použitím imunoglobulinu M v limitním ředění

Czech description

Byl vyvinut piezoelektrický imunosensor pro detekci patogenního mikroorganismu Francisella tularensis, působícího onemocnění tularemii. Analyzovaný vzorek se smíchá s velmi malým množstvím specifického IgM a vytvořený imunokomplex je změřen piezosensoremmodifikovaným tularemickým antigenem. Doba stanovení je 35 minut, mez detekce 5x10^6 bakterií.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/OBVTUO2003001" target="_blank" >OBVTUO2003001: Imunosensors of CWA and BWA</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Letters

ISSN

0003-2719

e-ISSN

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Volume of the periodical

38

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

12

Pages from-to

411-422

UT code for WoS article

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EID of the result in the Scopus database

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