Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022210" target="_blank" >RIV/00216224:14310/07:00022210 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Rapid Characterization of Monoclonal Antibodies using the Piezoelectric Immunosensor

Original language description

Monoclonal antibodies with specificity against the Francisella tularensis outerlipopolysaccharide (LPS) membrane were prepared and characterized using the piezoelectric immunosensor with immobilized LPS antigen from F. tularensis. Signals obtained by theimmunosensor were compared with ELISA and similar sensitivity was noticed. Signal of negative controls obtained using the biosensor was below 0.5% of the signal obtained for the selected specific antibody clone 4H3B9D3. Testing of cross reactivity basedon the sensors with immobilized LPS from Escherichia coli and Bacillus subtilis confirmed selectivity of this antibody. Furthermore, the 4H3B9D3 antibody was successfully isotypized as IgM using the piezoelectric sensors with secondary antibodies. Kinetics parameters of antibody were evaluated in the flow-through arrangement. The kinetic rate constants for the antibody 4H3B9D3 were ka = (2.31 +- 0.20)x10^5 l mol-1s-1 (association) and kd = (0.0010 +- 0.00062) s-1 (dissociation) indicati

Czech name

Rychlá charakterizace monoklonálních protilátek použitím piezoelektrického imunosensoru

Czech description

Byly hledány mAb specifické proti lipopolysacharidové složce bakterie Francisella tularensis pomocí piezoelektrického imunosensoru, nalezený klon byl kineticky charakterizován.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

341-353

UT code for WoS article

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EID of the result in the Scopus database

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