Label-free piezoelectric immunosensor for rapid assay of Escherichia coli

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00000493%3AG41__%2F08%3A%230000251" target="_blank" >RIV/00000493:G41__/08:#0000251 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Label-free piezoelectric immunosensor for rapid assay of Escherichia coli
Original language description
A piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 106-109 CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycle including regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+-0.4 Hz for the sample containing 108 CFU/mL.
Czech name
Piezoelektrický imunosensor pro rychlé stanovení Escherichia coli
Czech description
Piezoelektrický sennsor s imobilizovanou polyklonální protilátkou byl použit pro detekci E. coli v koncentračním rozmezí 106-109 CFU/mL. Vzorky mohou být analyzovány za 4 minuty a měřící cyklus včetně regeneračního kroku trvá 10 minut.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/OSVTUO2006003" target="_blank" >OSVTUO2006003: BIODETEKCE - Research of the new methods related to detection as well as identification of warfare biological agents</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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