A label free piezoelectric immunosensor for rapid assay of Escherichia coli

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026654" target="_blank" >RIV/00216224:14310/08:00026654 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/08:00001992
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
A label free piezoelectric immunosensor for rapid assay of Escherichia coli
Original language description
piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of thepiezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 106-109 CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycleincluding regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+/-0.4 Hz for the sample containing 108 CFU/mL.
Czech name
Přímý piezoelektrický imunosensor pro rychlé stanovení Escherichia coli
Czech description
Pro rychlé stanovení (4 min) bakterie Escherichia coli byl zkonstruován piezoelektrický imunosensor.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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