Piezoelectric biosensors for detection of microorganisms
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00075645" target="_blank" >RIV/00216224:14740/14:00075645 - isvavai.cz</a>
Result on the web
<a href="http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34" target="_blank" >http://web2.mendelu.cz/af_239_nanotech/data/soubory/sbornik2014.pdf#page=34</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Piezoelectric biosensors for detection of microorganisms
Original language description
Piezoelectric biosensors provide fast, specific and economic way of detection for a wide range of analytes ranging from metabolites to microorganisms. Specific antibodies were immobilised on gold electrodes of piezoelectric crystal and the biosensor wasapplied for real-time detection of model microorganisms. Three nonpathogenic strains of Escherichia coli (BL21, DH5 alpha and K-12) were used because of easy cultivation and availability of antibodies. Several methods of antibody immobilisation were compared. The immobilisation of reduced antibody using Sulfo-SMCC was the most effective achieving the limit of detection (LOD) 10^5 CFU x mL-1. Active and passive operation modes of quartz crystal microbalance were compared concluding that for detection ofmicroorganisms, both approaches are practically identical. The developed biosensor was connected to the cyclone air sampler allowing detection of microorganisms disseminated in the form of aerosol inside bioaerosol chamber. The achieved L
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XIV. Pracovní setkání fyzikálních chemiků a elektrochemiků
ISBN
9788021068421
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
32-35
Publisher name
Masarykova univerzita
Place of publication
Brno
Event location
Brno
Event date
Jun 3, 2014
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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