Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00021384" target="_blank" >RIV/00216224:14310/05:00021384 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

Original language description

A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified during the screening of 2500 Arabidopsis individual insertion T2 lines. The deviations in growth and development of the mutant plantsappeared already at the initial growth. The germination was completely inhibited (about 30 to 40% of the T3 seeds in each line) or distinctly delayed in comparison with wt plants. The next development of the mutant seedlings varied further on agar medium. Complex phenotypic expression from post-embryonic lethality associated with abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes was observed within this individual mutant line. TheSouthern blot and segregation analysis revealed that the mutant plants contained one insert of T-DNA tightly linked to the mutant locus. To be sure that the ambiguous mutant phenotype was due to a mutation at a single locus, we have mapp

Czech name

Izolace a charakterizace nového mutanta v genu pro protein obsahující PPR motiv u Arabidopsis thaliana

Czech description

Skríningem 2500 T-DNA mutantů Arabidopsis thaliana byla zachycena a charakterizována mutace v novém genu pro protein obsahující PPR (pentatricopeptide repeat)motiv. Gen byl lokalizován na chromozomu 1 a byl izolován metodou inverzní PCR.

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005

ISBN

80-86778-16-9

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

46

Publisher name

Institute of Plant Molecular Biology ASCR

Place of publication

České Budějovice

Event location

České Budějovice

Event date

Jan 1, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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