Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00015893" target="_blank" >RIV/00216224:14310/06:00015893 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/06:00076399

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

Original language description

A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified among T-DNA insertion lines. Tagging of the At1g53330 gene caused the appearance of a semi-lethal mutation with a complex phenotypic expression from embryo lethality associated with the abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes. The PPR protein At1g53330.1 was predicted to be targeted to mitochondria by TargetP and MitoProt programs. Complementation analysis comfirmed that phenotype is a result of a single T-DNA integration. A thorough functional analysis of this mutant aimed at finding a particular organelle target of At1g53330.1 protein will follow.

Czech name

Izolace a charakterizace nové semiletální mutace Arabidopsis thaliana v genu pro protein obsahující pentatricopeptide (PPR) motiv

Czech description

Mezi T-DNA inzerčními liniemi Arabidopsis thaliana byl identifován mutant v genu z rodiny pentatricopeptide repeat (PPR). Inzerce v genu At153330 způsobila semiletální mutaci s komplexním fenotypovým projevem včetně embryonální letality. Protein PPR je transportován do mitochondrií. Komplementační analýza potvrdila, že mutace je způsobena jedním inzertem T-DNA.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genetica

ISSN

0016-6707

e-ISSN

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Volume of the periodical

128

Issue of the periodical within the volume

1-3

Country of publishing house

LV - LATVIA

Number of pages

13

Pages from-to

395-407

UT code for WoS article

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EID of the result in the Scopus database

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