Unusual Entropy Driven Affinity of Chromobacterium violaceum Lectin CV-IIL towards Fucose and Mannose

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00015708" target="_blank" >RIV/00216224:14310/06:00015708 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/06:00016201

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Unusual Entropy Driven Affinity of Chromobacterium violaceum Lectin CV-IIL towards Fucose and Mannose

Original language description

The purple pigmented bacterium Chromobacterium Violaceum is a dominant component of tropical soil microbiota that can cause rare but fatal septicaemia in humans. Its sequenced genome provides insight into the abundant potential of this organism for biotechnological and pharmaceutical applications and allowed an ORF encoding a protein that is 60% identical to the fucose binding lectin (PA-IIL) from Pseudomonas aeruginosa and the mannose binding lectin (RS-IIL) from Ralstonia solanacearum to be identified. The lectin, CV-IIL, has recently been purified from C. Violaceum [Zinger-Yosovich, K., Sudakevitz, D., Imberty, A., Garber, N. C., and Gilboa-Garber, N. (2006) Microbiology 152, 457-463] and has been confirmed to be a tetramer with subunit size of 11.86 kDa and a binding preference for fucose. We describe here the cloning of CV-IIL and its expression as a recombinant protein. A complete structure-function characterization has been made in an effort to analyze the specificity and affini

Czech name

Neobvykle entropicky rizena affinita lektinu z Chromobacterium violaceum k fukose a manose

Czech description

Neobvykle entropicky rizena affinita lektinu z Chromobacterium violaceum k fukose a manose

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GD204%2F03%2FH016" target="_blank" >GD204/03/H016: Structural biophysics of macromolecules</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemistry

ISSN

0006-2960

e-ISSN

—

Volume of the periodical

45

Issue of the periodical within the volume

24

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

7501-7510

UT code for WoS article

—

EID of the result in the Scopus database

—