Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016803" target="_blank" >RIV/00216224:14310/06:00016803 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/06:00047932

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides

Original language description

Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents and conserves the diffusion current. Since for an adsorbed electroactive substance this elimination function yields a well readable peak-counterpeak signal, theadsorptive stripping (AdS) procedure was favorably used. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. Our resul

Czech name

Aplikace eliminační voltametrie pro rozlišení signálů adeninu a cytosinu v oligonukleotidech. II. Hetero-oligodeoxynukleotidy s různou sekvencí adeninových a cytosinových nukleotidů

Czech description

Aplikace eliminační voltametrie pro rozlišení signálů adeninu a cytosinu v oligonukleotidech. II. Hetero-oligodeoxynukleotidy s různou sekvencí adeninových a cytosinových nukleotidů.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

18

Issue of the periodical within the volume

7

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

662-669

UT code for WoS article

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EID of the result in the Scopus database

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