NMR 13C-relaxation Study of Base and Sugar Dynamics in GCAA RNA Hairpin Tetraloop

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00020606" target="_blank" >RIV/00216224:14310/07:00020606 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/07:00089118 RIV/60076658:12310/07:00008003

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

NMR 13C-relaxation Study of Base and Sugar Dynamics in GCAA RNA Hairpin Tetraloop

Original language description

Intramolecular dynamics of a 14-mer RNA hairpin including GCAA tetraloop was investigated by 13C NMR relaxation. R1 and R1rho relaxation rates were measured for all protonated base carbons as well as for C1' carbons of ribose sugars at several magnetic field strengths. The data has been interpreted in the framework of modelfree analysis (G. Lipari and A. Szabo, J. Am. Chem. Soc. 104, 4546-4559 (1982); G. Lipari and A. Szabo, J. Am. Chem. Soc. 104, 4559-4570 (1982)) characterizing the internal dynamics of the molecule by order parameters and correlation times for fast motions on picosecond to nanosecond time scale and by contributions of the chemical exchange. The fast dynamics reveals a rather rigid stem and a significantly more flexible loop. The cytosine and the last adenine bases in the loop as well as all the loop sugars exhibit a significant contribution of conformational equilibrium on microsecond to millisecond time scale. The high R1rho values detected on both base and sugar mo

Czech name

Studium dynamiky bazí a cukrů v RNA smyčce GCAA pomocí NMR relaxace C-13

Czech description

Práce popisuje výsledky studia intramolekulární dynamiky RNA vlásenky sestávající ze 14 nukleotidů a obsahující smyčku GCAA pomocí NMR relaxace jader C-13.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biomolecular Structure and Dynamics

ISSN

0739-1102

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

19

Pages from-to

307-326

UT code for WoS article

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EID of the result in the Scopus database

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