Characterization of Lactococcus lactis subsp. lactis isolated from surface waters

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022761" target="_blank" >RIV/00216224:14310/07:00022761 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Characterization of Lactococcus lactis subsp. lactis isolated from surface waters

Original language description

A group of nine strains isolated on Slanetz-Bartley agar and/or Kanamycin esculin azide agar were isolated as presumptive enterococci during a screening of Enterococcus spp. in surface waters. All analysed strains were clearly separated using rep-PCR with the (GTG)5 primer from all enterococci included in the in-house CCM database but they formed a homogeneous cluster matching Lactococcus lactis subsp. lactis representatives. Further identification using extensive biotyping as well as automated ribotyping with EcoRI (RiboPrinter microbial characterization system) confirmed all strains as L. lactis subsp. lactis. Obtained results showed that L. lactis subsp. lactis can be commonly isolated from different surface waters. These strains can be confused with enterococci due to their good growth on enterococcal selective media and their similarity in key phenotypical tests. (GTG)5-PCR revealed as a reliable and fast method for identification of L. lactis subsp.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů