Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00022879" target="_blank" >RIV/00216224:14310/07:00022879 - isvavai.cz</a>

Alternative codes found

RIV/00000493:G41__/07:#0000250

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

Original language description

Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day,it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.

Czech name

Serologické stanovení tularemie u myší použitím amperometrického imunosensoru

Czech description

Bylo detekováno množství anti tularemických protilátek v séru myší infikovaných bakterií Francisella tularensis, pro stanovení byl využit amperometrický imunosensor na bázi sítotiskové elektrody.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/OSVTUO2006003" target="_blank" >OSVTUO2006003: BIODETEKCE - Research of the new methods related to detection as well as identification of warfare biological agents</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

23

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

2507-2512

UT code for WoS article

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EID of the result in the Scopus database

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