Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00023007" target="_blank" >RIV/00216224:14310/07:00023007 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Characterization of Staphylococcus sp. deposited in the Czech Collection of Microorganisms
Original language description
The aim of this study was to clarify taxonomic position of a group of 29 biochemically presumptively identified Staphylococcus strains deposited in the Czech Collection of Microorganisms (http://www.sci.muni.cz/ccm/). Biotyping was done by API Staph, APIZym commercial kits and conventional tests. More detailed characterization was performed by ribotyping with both EcoRI and HindIII restriction endonucleases, whole-cell protein analysis and the (GTG)5-PCR fingerprinting. All applied methods congruentlyseparated most of analysed strains and assigned them as S. haemolyticus, S. equorum, S. saprophyticus subsp. saprophyticus, S. kloosii, S. cohnii subsp. cohnii and S. epidermidis species. However these methods did not provide congruent results in severalstrains. Ribotyping as well as protein analysis failed to unambiguously distinguish some oxidase positive S. sciuri, S. fleurettii and S. lentus strains revealing highly similar patterns; but they were clearly separated by the (GTG)5-PCR
Czech name
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Czech description
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Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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