MALDI TOF mass spectrometry of selected mycotoxins in barley

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00024765" target="_blank" >RIV/00216224:14310/07:00024765 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

MALDI TOF mass spectrometry of selected mycotoxins in barley

Original language description

A novel method for the simultaneous determination of the trichothecene mycotoxins deoxynivalenol (DON), nivalenol (NIV), 3-acetyl-deoxynivalenol (3-ADON), and 15-acetyl-deoxynivalenol (15-ADON) in barley and malt extracts has been developed using MatrixAssisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI TOF MS). This technique enables highly sensitive and fast analysis and/or detection using very small samples. In this work several matrices were examined and the most suitable ones were identified. Statistical analysis was carried out to verify the ability of the system to determine the mycotoxins in a real sample. Test for the accuracy of the method, repeatability, limit of detection (LOD) and limit of quantification (LOQ) were studied. Moreover, the accuracy of the method was confirmed by comparing analytical data to certified values from reference materials for those mycotoxins. In addition, a comparison of the analytical parameters for the determination of

Czech name

MALDI TOF hmotnostní spektrometrie vybraných mykotoxinů v obilce ječmene

Czech description

Metoda MALDI TOF MS byla použita pro stanovení/identifikaci nízkomolekulárních látek - trichothecenových mykotoxinů (deoxynivalenol, nivalenol, 3 a 15-acetyl-deoxynivalenol) - v obilce ječmene a sladu. Jednotlivé matrice (2,5-dihydroxybenzoová kyselina,0,2% hydrazin hydrát, suspenze diamantu v acetonitrilu a azidu v acetonitrilu) byly statisticky vyhodnoceny. Provedeny byly následující testy: přesnost stanovení molekulové hmotnosti, opakovatelnost, rozlišení, LOD a LOQ. Jako nejlepší matrice byly stanoveny suspenze azidu a diamantu v acetonitrilu. Metoda byla aplikována na reálné vzorky.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F04%2F2105" target="_blank" >GA203/04/2105: New procedures of barley and malt chemical analysis, determination of compounds responsible for their quality and chemometrics data exploration</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Biomedicine

ISSN

1214-0287

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

January

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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