Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F07%3A00050868" target="_blank" >RIV/00216224:14310/07:00050868 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/elan.200603738" target="_blank" >http://dx.doi.org/10.1002/elan.200603738</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.200603738" target="_blank" >10.1002/elan.200603738</a>

Result language

angličtina

Original language name

Determination of apo-metallothionein using adsorptive transfer stripping technique in connection with differential pulse voltammetry

Original language description

The main aim of this work was to prepare and determine apoMT by adsorptive transfer stripping technique (AdTS) in connection with differential pulse voltammetry (DPV). Particularly, we investigated the electrochemical behavior of MT measured on the surface of hanging mercury drop electrode in the presence of sodium chloride as supporting electrolyte using AdTS DPV with respect to study the effects of MT signals by different concentrations and pH's of the electrolyte. Then, we aimed at utilizing this technique to observe changes in MTwhich are dependent on using strong chelating compounds ethylendiamine-N, N, N', N'-tetraacetic acid (EDTA). Thanks to ability of EDTA to bind heavy metals from active center of enzymes and regulation proteins, we were ableto prepare apoMT; that means, MT without any metal ion bound in its structure.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

2-3

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

339-347

UT code for WoS article

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EID of the result in the Scopus database

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