Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00024771" target="_blank" >RIV/00216224:14310/08:00024771 - isvavai.cz</a>

Alternative codes found

RIV/60193697:_____/08:#0000440

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of Ergosterol Levels in Barley and Malt Varieties in the Czech Republic via HPLC

Original language description

In this study ergosterol levels in different varieties of barley and malt produced in the Czech Republic were determined. A modified high performance liquid chromatography (HPLC) method was statistically processed, validated (Effivalidation program) andapplied to 124 samples of barley and malt. Ergosterol was isolated by extraction, saponification and the quantification was performed using HPLC with diode array detection (DAD). The content of ergosterol ranged between LOD-36.3 mg/kg in barley and LOD-131.1 mg/kg in malt. Ergosterol is presumably connected with metabolites generated when barley grain is attacked by pathogens and such barley often shows a high overfoaming (gushing) value. However, it was found that the content of ergosterol does not correlate with the degree of beer gushing.

Czech name

Stanovení ergosterolu v odrůdách ječmene a sladu v České republice pomocí HPLC

Czech description

V této práci byl stanovován obsah ergosterolu v odrůdách ječmene a sladu pěstovaných v ČR metodou HPLC. Tato metoda byla statisticky vyhodnocena, validována a aplikována na 124 vzorků ječmene a sladu. Ergosterol byl izolován extrakcí, saponifikací a kvantifikován pomocí HPLC s DAD. Obsah ergosterolu se pohyboval mezi LOD-36.3 mg/kg v ječmeni a LOD-131.1 mg/kg ve sladu. Ergosterol je spojován s metabolity produkovanými pokud je ječmenné zrno napadeno pathogeny.Ječmen pak vykazuje vysokou hodnotu přepěňování. Přesto bylo zjištěno, že obsah ergosterolu nekoreluje se stupněm přepěňování piva.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA525%2F06%2F0663" target="_blank" >GA525/06/0663: Study of the chemical changes of barley caryopses after an attack by pathogens to monitor and improve the quality of barley and its derived food produ</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

4

Pages from-to

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UT code for WoS article

000256445300029

EID of the result in the Scopus database

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