Evaluation of the possible proteomic application of trypsin from Streptomyces griseus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00025879" target="_blank" >RIV/00216224:14310/08:00025879 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/08:00312520 RIV/61989592:15310/08:00005532

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Evaluation of the possible proteomic application of trypsin from Streptomyces griseus

Original language description

In this work, trypsin from Streptomyces griseus (SGT) was purified to homogeneity and evaluated in in-gel digestion of protein standards followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses of the digests. Thenumber of produced and matching tryptic peptides was higher than in the case of commonly used bovine trypsin and allowed us to obtain higher identification scores in database searches. Interestingly, SGT was found to also generate nonspecific peptides,a partial F-X, Y-X, and W-X cleavage specificity. To suppress autolysis, either arginine or arginine plus lysine residues in SGT were modified by chemical reagents. In consequence, the autolytic pattern of SGT was reduced significantly, but specific activity dropped dramatically. As demonstrated by relative quantification, SGT is more stable at 37 degs than is its bovine counterpart. We conclude that SGT represents a convenient alternative for proteomic applications involving protein dig

Czech name

Zjištění možných proteomických uplatnění tryspinu ze Streptomyces griseus

Czech description

V této práci byla popsána izolace a charakteriza trypsinu ze Streptomyces griseus pro použití v proteomice.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/LC06034" target="_blank" >LC06034: Regulation of morphogenesis of plant cells and organs</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Biochemistry

ISSN

0003-2697

e-ISSN

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Volume of the periodical

376

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

000255104100009

EID of the result in the Scopus database

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