Sensitivity analysis of RecA genotoxicity test

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026345" target="_blank" >RIV/00216224:14310/08:00026345 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Sensitivity analysis of RecA genotoxicity test
Original language description
RecA test is a short-term luminescent SOS genotoxicity test. The induction of the SOS system is detected through the activation of the reporter gene system - DNA damage SOS repair system. The test is based on the swift elevation of recA gene expression in response to DNA damage. In case of RecA test there is a specific recA::luxCDABE gene fusion. It is fusion of reporter gene and specific gene of SOS repair system. This gene fusion could detect genotoxicity efficiently. In this study Escherichia coli strains DPD2794, DPD2797, DPD1718 and DPD1710 were used for detection of genotoxicity. The genotoxicity was detected by monitoring of bioluminescent emission. For assessment of the sensitivity evaluation a group of chemicals and environmental samples wereselected. The aim of this research was to decide whether this test has future applications in the screening evaluation of genotoxic potential and to compare sensitivity of the RecA test with various strains of Escherichia coli. Results in
Czech name
viz. angl.
Czech description
viz. angl.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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