Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F20%3A00114583" target="_blank" >RIV/00216224:14310/20:00114583 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/20:00073504
Result on the web
<a href="https://doi.org/10.1038/s41598-020-76426-2" target="_blank" >https://doi.org/10.1038/s41598-020-76426-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-020-76426-2" target="_blank" >10.1038/s41598-020-76426-2</a>

Result language
angličtina
Original language name
Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability
Original language description
Extracellular pH has been assumed to play little if any role in how bacteria respond to antibiotics and antibiotic resistance development. Here, we show that the intracellular pH of Escherichia coli equilibrates to the environmental pH following treatment with the DNA damaging antibiotic nalidixic acid. We demonstrate that this allows the environmental pH to influence the transcription of various DNA damage response genes and physiological processes such as filamentation. Using purified RecA and a known pH-sensitive mutant variant RecA K250R we show how pH can affect the biochemical activity of a protein central to control of the bacterial DNA damage response system. Finally, two different mutagenesis assays indicate that environmental pH affects antibiotic resistance development. Specifically, at environmental pH's greater than six we find that mutagenesis plays a significant role in producing antibiotic resistant mutants. At pH's less than or equal to 6 the genome appears more stable but extensive filamentation is observed, a phenomenon that has previously been linked to increased survival in the presence of macrophages.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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