A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026700" target="_blank" >RIV/00216224:14310/08:00026700 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

A determination of metallothionein in larvae of freshwater midges (Chironomus riparius) using Brdicka reaction

Original language description

Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothioneinand total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from field studies using differential pulse voltammetry Brdicka reaction. Unique electrochemical instrument, stationaryelectrochemical analyser Autolab coupled with autosampler, was utilized for the analysis of the samples. The detection limit for MT was evaluated as 5 nM. The larvae exposed to two doses of cadmium(II) ions for fifteen days under laboratory controlled conditions were at the end of the exposure killed, homogenized and analysed. MT content in control samples was 1.2 microM, in larvae exposed to 50 ng Cd/g it was 2.0 microM and in larvae exposed to 50 microg Cd/g 2.9 microM. Moreover at fi

Czech name

Stanovení metalothioneinu v larvách pakomára (Chironomus riparius) s využitím Brdičkovy reakce

Czech description

Mezi biomolekuly, které odpovídají na stres, patří metalothioneiny, vhodné pro sledování kontaminace těžkými kovy. Cílem této práce bylo stanovit obsah metalothioneinů a celkových thiolů v larvách pakomárů exponovaných kadmiem a přírodními sedimenty.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors

ISSN

1424-8220

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

—

UT code for WoS article

000258180500003

EID of the result in the Scopus database

—