Phytochelatin synthase activity as a marker of metal pollution

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F11%3A43870544" target="_blank" >RIV/62157124:16270/11:43870544 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/11:00176233 RIV/00216305:26620/11:PU95050
Result on the web
<a href="http://dx.doi.org/10.1016/j.jhazmat.2011.05.088" target="_blank" >http://dx.doi.org/10.1016/j.jhazmat.2011.05.088</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jhazmat.2011.05.088" target="_blank" >10.1016/j.jhazmat.2011.05.088</a>

Result language
angličtina
Original language name
Phytochelatin synthase activity as a marker of metal pollution
Original language description
The synthesis of phytochelatins is catalyzed by gama-Glu-Cys dipeptidyl transpeptidase called phytochelatin synthase (PCS). Aim of this study was to suggest a new tool for determination of phytochelatin synthase activity in the tobacco BY-2 cells treatedwith different concentrations of the Cd(II). After the optimization steps, an experiment on BY-2 cells exposed to different concentrations of Cd(NO(3))(2) for 3 days was performed. At the end of the experiment, cells were harvested and homogenized. Reduced glutathione and cadmium (II) ions were added to the cell suspension supernatant. These mixtures were incubated at 35°C for 30min and analysed using high performance liquid chromatography coupled with electrochemical detector (HPLC-ED). The results revealed that PCS activity rises markedly with increasing concentration of cadmium (II) ions. The lowest concentration of the toxic metal ions caused almost three fold increase in PCS activity as compared to control samples. The activity of
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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