Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00174192" target="_blank" >RIV/62156489:43210/11:00174192 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Employment of HPLC with coulometric detection for analysis of phytochelatin synthase activity
Original language description
Phytochelatins are physiologically active peptides which have to be synthesized by phytochelatin synthase (PCS). The main aim of this study was to optimize high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) as suitabletool for determination of the phytochelatin synthase activity. Coulometric detector with porous graphite working electrode was used. Firstly we optimized a separation of two compounds which are important for monitoring of PCS activity. It was glutathione reduced as substrate for the reaction and fytochelatin-2 as a product. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various concentrations of cadmium. We then homogenized the cells and immediately analyzed the extracts by optimized HPLC-ED method. Moreover we observed that with higher concentrations of applied cadmium there was increasing of amount of PC-2. The
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XI. pracovní setkání fyzikálních chemiků a elektrochemiků
ISBN
978-80-7375-514-0
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
332-335
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
MENDELU
Event date
Jan 1, 2011
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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