A novel method for analysis of plants' resistance to heavy metals

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00180218" target="_blank" >RIV/62156489:43210/11:00180218 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2011/mendelnet_2011_fulltext.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
A novel method for analysis of plants' resistance to heavy metals
Original language description
Nowadays many less developed countries has many problems concerning about pollution in environment caused by heavy metals as cadmium, lead or mercury. The main aim of this work was to optimize method for determination of activity of main plant protectivemechanism against the heavy metals. Sulphur rich peptides phytochlatins (PC's) are physiologically active compounds because they are able of immobilization of the toxic heavy metal directly in the plant. Most occurring of PC's is phytochelatin2 (PC-2) which is synthesized by phytochelatin synthase (PCS). We focused on developing of the method based on high performance liquid chromatography coupled with electrochemical detector (HPLC-ED) for determination of PC-2 because it might serve as suitable toolfor determination of phytochelatin synthase activity. PCS is the best activated by cadmium ions. We used a model with BY-2 tobacco cells. We conducted the in vivo 3 day cultivation experiment where BY-2 cells were treated by various conce
Czech name
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Czech description
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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