Multidetection platform for microcolumn separation of proteins and peptides

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00033542" target="_blank" >RIV/00216224:14310/08:00033542 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Multidetection platform for microcolumn separation of proteins and peptides
Original language description
This lecture will describe an instrumentation platform based on universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-line using MALDI mass ortandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. Another detection mode is inductively coupled plasma optical emission and/ormass spectrometry (ICP OES, resp. MS), nature of which is complementary to soft MALDI MS. For the first time, we proposed MALD for desorption of sample from the target and introduction to ICP. Thus, both information about protein identity and content ofelements, such as metals or phosphorus will be available from a single separation record. Initial results of MALD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new platform.
Czech name
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Czech description
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Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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