A Multidetection Platform for Proteome Analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00058776" target="_blank" >RIV/00216224:14310/08:00058776 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
A Multidetection Platform for Proteome Analysis
Original language description
This lecture will describe an instrumentation platform based on a universal interface for deposition of capillary electrophoresis (CE) eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides are analyzed off-lineusing MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions; such as enzymatic digestion. Another detection mode is inductively coupled plasma mass spectrometry (ICPMS), which is complementary to soft MALDI MS. We propose substrate-enhanced laser desorption (SELD) for transfer of deposited sample from the target to ICP. Thus, both information about protein identity and content of elements, such as metals or phosphorus is available from a single separation record. Initial results of SELD - ICP OES/MS analysis of metal species will be shown to demonstrate the feasibility of the new approach.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů