Interaction of cryptogein with its binding sites in tobacco plasma

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029172" target="_blank" >RIV/00216224:14310/09:00029172 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Interaction of cryptogein with its binding sites in tobacco plasma
Original language description
Elicitins are low molecular weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, this process is triggered by binding of elicitin to the high affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild type and mutant forms) were covalently immobilized on the sensing surface and membrane vesicles s containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association kas = 5.74x10E6 M1 s-1 and dissociation kdis = 6.87x10-4 s-1 constants, respectively. The kinetic equilibrium dissociation constant was calculated, KD = 12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/GA522%2F06%2F0156" target="_blank" >GA522/06/0156: Structure-function relationship of elicitors from Phytophthora spp.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)