Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029473" target="_blank" >RIV/00216224:14310/09:00029473 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Substrate-Assited Laser Desorption Inductively-Coupled Plasma Mass Spectrometry for Elemental Detection in Microcolumn Effluent and Biological Submicroliter Samples
Original language description
A new method of sample preparation for elemental analysis using substrate-assisted laser desorption inductively-coupled plasma mass spectrometry (SALD ICP MS) is presented. Well-defined submicroliter volumes of analyzed samples are deposited onto polyethylene terephthalate glycol (PETG) plate and, after insertion into a commercial ablation cell, desorbed with pulses of 213-nm laser and analyzed with ICP MS. In contrast to conventional laser ablation (LA) ICP MS of solids, the analyzed compound is quantitatively desorbed during the PETG ablation. Furthermore, complete desorption is achieved at laser power density as low as ~10 MW/cm2, two orders below the levels typical in LA ICP MS. Model samples of metal salts in pg quantities were desorbed by less than 20 laser shots and relative spot-to-spot reproducibility was 3-10%. The limits of detection of Cr, Cu, Co, Fe, Ni, Sn and Zn deposited on the target were in the range of 50-500 fg.
Czech name
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Czech description
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Project
<a href="/en/project/GA203%2F09%2F1025" target="_blank" >GA203/09/1025: Multidetection platform for microcolumn separation of proteins and peptides</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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