Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of biological microsamples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044535" target="_blank" >RIV/00216224:14310/10:00044535 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of biological microsamples
Original language description
A new method for determination of trace elements in submicroliter sample volumes using substrate-assisted laser desorption inductively-coupled plasma mass spectrometry (SALD ICP MS ) is presented. In contrast to conventional laser ablation (LA) ICP MS, SALD ICP MS allows quantitative analysis of metals in liquid samples because of complete sample desorption, which can be achieved using a few pulses with spot-to-spot reproducibility below 6 %. Detailed characterization, such as laser power density, raster and point desorption optimization etc. will be discussed. The performance of off-line coupling capillary electrophoresis and SALD ICP was demonstrated on speciation of 1 pg of Cr(III) and Cr (VI). SALD ICP MS can be used for the determination of traceelements in minute amounts of biological samples at physiological level as demonstrated on the determination of copper in myeloid leukemia cells to investigate the role of copper ions in cytotoxicity regulation with disulfiram .
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F09%2F1025" target="_blank" >GA203/09/1025: Multidetection platform for microcolumn separation of proteins and peptides</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Sborník 14. česko ? slovenské spektroskopické konference
ISBN
978-80-7395-282-2
ISSN
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e-ISSN
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Number of pages
149
Pages from-to
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Publisher name
Spektroskopická společnost Jana Marka Marci
Place of publication
Pardubice
Event location
Litomyšl
Event date
Jan 1, 2010
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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