Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of copper in myeloid leukemia cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00043957" target="_blank" >RIV/00216224:14310/10:00043957 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of copper in myeloid leukemia cells

Original language description

This work presents a detailed characterization of substrate-assisted laser desorption inductively coupled plasma mass spectrometry (SALD ICP-MS), a new approach in the determination of trace elements in submicrolitre volumes. SALD ICP-MS allows quantitative analysis of metals in liquid samples with defined volumes deposited onto an absorbing substrate due to complete sample desorption at relatively low laser power density. Analysis of model samples of Cu(II) showed a constant signal over a wide range oflaser power density, an independence of signal on desorption modes and a wide linear dynamic range. SALD ICP-MS with its limit of detection for copper below 26 fg in submicrolitre samples was successfully applied to investigate cytotoxic effects of disulfiram in human myeloid leukemia cells U937 depending on intracellular levels of copper.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

<a href="/en/project/GA203%2F09%2F1025" target="_blank" >GA203/09/1025: Multidetection platform for microcolumn separation of proteins and peptides</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY

ISSN

0267-9477

e-ISSN

—

Volume of the periodical

25

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—