Identification of genes participating in the regulation of CKI1 expression using forward genetic approach

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029985" target="_blank" >RIV/00216224:14310/09:00029985 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Identification of genes participating in the regulation of CKI1 expression using forward genetic approach
Original language description
Overexpression of a hybrid histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling pathway and cytokinin-like response in Arabidopsis hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of twocomponent signaling. We used forward genetics approach to identify the factors regulating theactivity of CKI1 promoter. Stable transgenic lines carrying ProCKI1:uidA construct were mutated using EMS and M2 seedlings were screened for changes in the GUS pattern. To eliminate constraints of the rate-limiting factor that is a microscopy of the screened mutant lines, we have adopted automated microscopy using the dotSlide system. The level of CKI1 gene expression in mutant lines was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress.
Czech name
—
Czech description
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)