IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00029986" target="_blank" >RIV/00216224:14310/09:00029986 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESSION USING FORWARD GENETIC APPROACH
Original language description
Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development andprocambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based clon
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us"
ISBN
978-80-7375-310-8
ISSN
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e-ISSN
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Number of pages
28
Pages from-to
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Publisher name
Mendel University of Agriculture and Forestry in Brno
Place of publication
Brno
Event location
Mendel University of Agriculture and Forestry in
Event date
Jun 30, 2009
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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