Separation of PCR-ready DNA from dairy products using magnetic hydrophilic microspheres and poly(ethylene glykol)-NaCl water solutions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00036210" target="_blank" >RIV/00216224:14310/09:00036210 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/09:PU82037
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Separation of PCR-ready DNA from dairy products using magnetic hydrophilic microspheres and poly(ethylene glykol)-NaCl water solutions
Original language description
Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co- glycidyl dimethylmethacrylate) (P(HEMA-co-GMA)) and magnetic glass microspheres were used for the isolation of bacterial DNA. The most extensive DNA recovery achieved at PEG 6000 concentrations of 12% or 16 % and 2 M NaCl. The method proposed was used for bacterial DNA isolation from different dairy products containing Bifidobacterium and Lactobacillus cells. The presence of target DNA and quality of isolated DNA was checked by PCR amplification with specific primers.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Magnetism and Magnetic Materials
ISSN
0304-8853
e-ISSN
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Volume of the periodical
321
Issue of the periodical within the volume
10
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
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UT code for WoS article
000265278000076
EID of the result in the Scopus database
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