Capture of DNAs by magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F21%3A00537230" target="_blank" >RIV/61389013:_____/21:00537230 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/21:00537230 RIV/00216305:26310/21:PU144082
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs10853-020-05649-5" target="_blank" >https://link.springer.com/article/10.1007%2Fs10853-020-05649-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10853-020-05649-5" target="_blank" >10.1007/s10853-020-05649-5</a>
Alternative languages
Result language
angličtina
Original language name
Capture of DNAs by magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres
Original language description
Magnetic hypercrosslinked poly(styrene-co-divinylbenzene) microspheres (mgt.HPS1-NH2 and mgt.HPS2-NH2) containing different contents of amino groups were prepared and characterized in this study. The microspheres were used for the capture of uncompacted and compacted bacterial and calf thymus DNAs in the presence of different PEG 6000 and NaCl concentrations. Magnetic macroporous poly(glycidyl methacrylate-co-[2-(methacryloyloxy)ethoxy]acetic acid-co-ethylene dimethacrylate) microspheres containing amino and carboxyl groups [mgt.P(GMA-MOEAA-EDMA)-NH2] and magnetic non-porous carboxyl group-functionalized poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [mgt.P(HEMA-GMA)-COOH] microspheres were used as a control. The size changes of uncompacted and compacted bacterial and calf thymus DNAs were measured in the range of concentrations 0–16% PEG 6000 and 0.5–2.0 M NaCl by dynamic light scattering (DLS). The highest capture of compacted bacterial and calf thymus DNAs was achieved with mgt.HPS1-NH2, mgt.HPS2-NH2 microspheres using 8% PEG 6000 and 2.0 M NaCl and with mgt.P(GMA-MOEAA-EDMA)-NH2 microspheres using 8% and 16% PEG 6000 and 2.0 M NaCl. Developed microspheres were used for DNA isolation from real vegetable (broccoli flowering head) samples. DNA was in quality suitable for PCR.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Materials Science
ISSN
0022-2461
e-ISSN
1573-4803
Volume of the periodical
56
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
5817-5829
UT code for WoS article
000604558800036
EID of the result in the Scopus database
2-s2.0-85098571721