Distinct roles of non-canonical poly(A) polymerases in RNA metabolism

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00036231" target="_blank" >RIV/00216224:14310/09:00036231 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Distinct roles of non-canonical poly(A) polymerases in RNA metabolism

Original language description

Trf4p and Trf5p are non canonical poly(A) polymerases and are part of the heteromeric protein complexes TRAMP4 and TRAMP5 that promote the degradation of aberrant and short lived RNA substrates by interacting with the nuclear exosome. To assess the levelof functional redundancy between the paralogous Trf4 and Trf5 proteins and to investigate the role of the Trf4 dependent polyadenylation in vivo, we used DNA microarrays to compare gene expression of the wild-type yeast strain of S. cerevisiae with either that of trf4delta or trf5delta mutant strains or the trf4delta mutant expressing the polyadenylation-defective Trf4(DADA) protein. We found little overlap between the sets of transcripts with altered expression in the trf4delta or the trf5delta mutants, suggesting that Trf4p and Trf5p target distinct groups of RNAs for degradation.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS Genetics

ISSN

1553-7390

e-ISSN

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Volume of the periodical

5

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

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UT code for WoS article

000269219500032

EID of the result in the Scopus database

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