Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F14%3A00073613" target="_blank" >RIV/00216224:14740/14:00073613 - isvavai.cz</a>
Result on the web
<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186968/" target="_blank" >http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186968/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molcel.2014.05.031" target="_blank" >10.1016/j.molcel.2014.05.031</a>
Alternative languages
Result language
angličtina
Original language name
Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation
Original language description
The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of thereleased transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Cell
ISSN
1097-2765
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
467-481
UT code for WoS article
000340646600012
EID of the result in the Scopus database
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