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Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F09%3A00039058" target="_blank" >RIV/00216224:14310/09:00039058 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216305:26310/09:PU82038

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Functionalised magnetic microsphereswith hydrophilic properties for molecular diagnostic applications

  • Original language description

    Magnetic nonporous hydrophilic microspheres poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate)-(P(HEMA-co-EDMA), poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate)-P(HEMA-co-GMA), and poly(glycidyl methacrylate)-PGMA were prepared by single-step dispersion polymerisation in the presence of sterically or electrostatically stabilised colloidal magnetite or maghemite. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres andtheir applicability in degradation of bacterial RNA, chromosomal and plasmid DNAs, degradation of intracellular PCR inhibitors and immunomagnetic separation of Salmonella cells were demonstrated. Heterogeneous lanthanide complexes of ethylenediaminetetraacetic acid (EDTA) immobilised on insoluble PGMA support and iminodiacetic acid (IDA) immobilised on magnetic P(HEMA-co-EDMA) microspheres were used as catalysts of plasmid DNA cleavage.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FOOD RESEARCH INTERNATIONAL

  • ISSN

    0963-9969

  • e-ISSN

  • Volume of the periodical

    42

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    6

  • Pages from-to

  • UT code for WoS article

    000266197300014

  • EID of the result in the Scopus database