Cloning and expression of endolysin and its SH3 domain from polyvalent staphylococcal bacteriophage

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044000" target="_blank" >RIV/00216224:14310/10:00044000 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cloning and expression of endolysin and its SH3 domain from polyvalent staphylococcal bacteriophage
Original language description
Endolysins are phage lytic proteins which digest the cell wall of bacteria. Here we report expression of the genes encoding lytic proteins from bacteriophage 812. This enzymes lyses cells of Staphylococcus aureus and could have practical use in phage therapy. Activity of lytic enzymes was tested on different S. aureus strains that are insensitive to wild-type phage 812 and are resistant to antibiotics. The enzymes were active against a broader range of bacterial strains than the phage itself. Lytic protein of phage 812 includes three domains. SH3-domain is probably cell wall targeting domain, which could be responsible for binding of endolysin to peptidoglycan. SH3b-domain of phage 812 has been cloned and expressed as a soluble protein in E. coli. Protein was purified to homogeneity using ammonium sulphate precipitation, anion exchange chromatography, cation exchange chromatography, and gel filtration.
Czech name
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Czech description
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Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>O - Projekt operacniho programu<br>R - Projekt Ramcoveho programu EK<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XIV. Setkání biochemiků a molekulárních biologů. Sborník příspěvků
ISBN
978-80-210-5164-5
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
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Publisher name
Masarykova univerzita
Place of publication
Brno
Event location
Konfernční centrum hotelu Continental v Brně
Event date
Apr 20, 2010
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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