RETENTION BEHAVIOUR OF OLIGONUCLEOTIDES ISOMERS STUDIED BY MEANS OF HYDROPHILIC INTERACTION CHROMATOGRAPHY

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00044249" target="_blank" >RIV/00216224:14310/10:00044249 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
RETENTION BEHAVIOUR OF OLIGONUCLEOTIDES ISOMERS STUDIED BY MEANS OF HYDROPHILIC INTERACTION CHROMATOGRAPHY
Original language description
Short oligonucleotides and its isomers are deeply studied for better understanding of nucleic acid properties and interactions. High performance liquid chromatography (HPLC) provides suitable tool for their characterization. Our interest in this study was focused on the separation of pentamers, consisted of adenine, guanine and thymine by means of hydrophilic liquid chromatography mode (HILIC). The influence of experimental parameters such as mobile phase pH and/or buffer concentration, percentage of organic solvent in mobile phase and flow rate was evaluated and the most acceptable conditions were selected for further analysis. Finally, obtained results were compared with commonly applied reverse phase liquid chromatography (RP HPLC).
Czech name
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Czech description
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Project
<a href="/en/project/LC06035" target="_blank" >LC06035: Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
ISBN
978-80-7375-396-2
ISSN
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e-ISSN
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Number of pages
2
Pages from-to
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Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Jun 23, 2010
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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