Strong cation- and zwitterion-exchange-type mixed-mode stationary phases for separation of pharmaceuticals and biogenic amines in different chromatographic modes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F21%3A39917849" target="_blank" >RIV/00216275:25310/21:39917849 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22310/21:43923663 RIV/60461373:22330/21:43923663 RIV/60461373:22810/21:43923663
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0021967320310256?token=8F186852B5243ED3F988C1B61312C2F4A60337B5DFA790C605D2AD97919292CFEA035E8AC00DADA318D779133CEA4612&originRegion=eu-west-1&originCreation=20211215132229" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0021967320310256?token=8F186852B5243ED3F988C1B61312C2F4A60337B5DFA790C605D2AD97919292CFEA035E8AC00DADA318D779133CEA4612&originRegion=eu-west-1&originCreation=20211215132229</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2020.461751" target="_blank" >10.1016/j.chroma.2020.461751</a>
Alternative languages
Result language
angličtina
Original language name
Strong cation- and zwitterion-exchange-type mixed-mode stationary phases for separation of pharmaceuticals and biogenic amines in different chromatographic modes
Original language description
A set of new mixed-mode ion-exchange stationary phases is presented. The backbone of organic selectors is formed by a linear hydrocarbon chain, which is divided into two parts of various lengths by a heteroatom (oxygen or nitrogen). In all studied cases, there is a sulfonic acid moiety as the terminal group. Therefore, selectors bearing oxygen gave rise to strong cation ion-exchange stationary phases, while selectors with an embedded nitrogen atom (inducing a weak anion exchange capacity) were used to create zwitterion ion-exchange stationary phases. The new mixed-mode stationary phases were chromatographically evaluated in high performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC) using isocratic elution conditions to disclose their chromatographic potential. In HPLC mode, aqueous-rich reversed phase chromatography, acetonitrile-rich hydrophilic interaction liquid chromatography and methanolic ion-exchange chromatography mobile phases were employed. In these chromatographic modes, retention factors and selectivity values for a test set of basic and zwitterionic analytes were determined. The results were compared and principal component analysis for each chromatographic mode was performed. For all chromatographic modes, the component 1 in the principal component analysis reflected the elution order. The application of different mobile phases on a particular column resulted not only in variation in retention, but also in modified selectivity, and different elution order of the analytes. The orthogonality of the elution order depending on the employed mobile phase conditions was especially reflected for structurally closely related analytes, such as melatonin and N-acetyl-serotonin, tryptamine and serotonin or noradrenalin and octopamine. However, ion-exchange interactions remain the main driving force for retention. From all investigated stationary phases, the SCX 2 (C5-linker and C4-spacer) seems to be the best choice for the separation of basic analytes using different mobile phase conditions. (C) 2020 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GJ20-23290Y" target="_blank" >GJ20-23290Y: Supercritical fluid chromatography hyphenated to mass spectrometry for the absolute quantitation of ionic and polar biomolecules</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9673
e-ISSN
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Volume of the periodical
1635
Issue of the periodical within the volume
January
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
461751
UT code for WoS article
000603564600016
EID of the result in the Scopus database
2-s2.0-85097330648